Coding

Part:BBa_K779315:Design

Designed by: Felix Sun   Group: iGEM12_MIT   (2012-09-29)


5' Hammerhead with loss-of-function mutation MammoBlock


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4
    Illegal BsaI.rc site found at 182


Design Notes

To be completed by MIT iGEM 2012.


Source

Sequence from Figure 1 of "Hammerhead ribozyme design and application", M. Amarzguioui and H. Prydz 1998.

References